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Description
| Biochem/physiol Actions | Casein kinases (CK) are highly conserved serine/threonine kinases, ubiquitous in eukaryotic organisms, and defined by their substrate preference for casein. They are insensitive to cyclic nucleotides, Ca2+, and phospholipids. Casein kinases are grouped into two main families, type I and type II, according to their structure, specificity, and response to effectors. |
| General description | The kit provides an easy method for measuring the activity of the different casein kinases by an in vitro phosphorylation of α-casein. The kit includes CK I and CK II specific inhibitors to distinguish between CK I and CK II activities in the biological sample. The kit can be used for CK activity measurement in cell lysates, tissue homogenates, column fractions, or of the purified enzyme |
Properties
| usage | sufficient for 100 tests |
| Gene Information | human ... CSNK1A1(1452), CSNK2A1(1457) |
| shipped in | dry ice |
| storage temp. | −20°C |
Components
| Kit component only | Assay Buffer for Casein Kinase Activity 5 x 1.5 mL |
| | ATP Solution 0.5 mL |
| | Casein Kinase Substrate (Alpha Casein) 1 mL |
| Kit component also available separately | Casein Kinase II Inhibitor (Heparin) 1 mL |
| | Casein Kinase I Inhibitor (IC 261) 0.5 mL |
| | Enzyme Dilution Buffer 1.7 mL |
References
| reference | Kuenzel, E.A., et al., Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides. J. Biol. Chem. 262, 9136-9140 , (1987) Abstract |
